Team Name: DDNA Red- Player Roster: Emma Cartwright, Simon Cartwright, Jamie Clark, Victoria Febbo, Ellen Hurley, Chris I’Anson, Chelsea Imanisani, Sam MacKerras, Ashlea Melnikas & Craig Moore
- Finish last year: Runner Up*
- Team Name: DDNA Teal
- Player Roster: Alec Deasey, Claire Garrick, Meg Hogan, Tyson Lingham, Nikki McKissack, Jake McSwain, Maddie Noble, Rob Rovetto, Gretel Schroder & Lani Ward
- Finish last year: Runner Up*
- Team Name: Fitzroy Phoenix
- Player Roster: Colin Croft, Jane Higgs, Daniel Kostovski, Billie Nukunuku, Kate Patterson, Ricky Papara, Jane Scheetz, Trent Williams & Sarah Winch
- Finish last year: Inaugural season
- Team Name: Melbourne Bucks Mixed
- Player Roster: Ryan Clements, Brianne Johnstone, Jessie Kapustic, Steve McCallum, Corey Mattson, Fi Prichard, Zoe Ryde, Paul Teys & Julia Woolley
- Finish last year: Inaugural season
- Team Name: Monash Palladium
- Player Roster: Caitlyn Ashby, Matthew Bird, Michael Dower, Samuel Dugan, Clare Kenyon, Laura Milton, Vanessa Redpath, Staci Tully & John Webby
- Finish last year: 4th
- Team Name: Northern Illusions
- Player Roster: Casie Beddome, Kris Buchen, Melissa Hutton, Alysha Hutton, Angel Mann, Ethan Mann, Trevor Mann, Kimmi Herum & Blaine Shugg
- Finish last year: 9th
- Team Name: Northern Titans
- Player Roster: Andrew Baker, Maggie Bromley, Ben Bruitzman, Aaron Clarke, Andrew Davis, Brent Holtham, Kate McCarthy, Briony Mack, Joanne Sanders & Simone Stewart
- Finish last year: 8th
- Team Name: Yarra Jets
- Player Roster: Sarah Brownell, Alex Green, Carly Hobday, Stace Jeffrey, Jamie-Anne O’Hare, Phil Pene, David Priddle, Michaela Russo & Kurt Thompson
- Finish last year: 7th
*DDNA Red and DDNA Teal competed as one team last season
